2011-09-26T16:53:39-07:00
Resource:PrimerBank
uncurated
http://pga.mgh.harvard.edu/primerbank/
<br />
An online database, PrimerBank, has been created for researchers to retrieve primer information for their genes of interest. PrimerBank currently contains 147,404 primers encompassing most known human and mouse genes. The primer design algorithm has been tested by conventional and real-time PCR for a subset of 112 primer pairs with a success rate of 98.2%.<br />
PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time PCR). PrimerBank contains over 306,800 primers covering most known human and mouse genes. There are several ways to search for primers: GenBank Accession, NCBI protein accession, NCBI Gene ID, Gene Symbol, PrimerBank ID or Keyword (gene description) or you can blast your gene sequence against the primerbank Sequence DB.<br />
The primer design algorithm has been extensively tested by real-time PCR experiments for PCR specificity and efficiency. We have tested 26,855 primer pairs that correspond to 27,681 mouse genes by Real Time PCR followed by agarose gel electrophoresis and sequencing of the PCR products. The design success rate is 82.6% (22,187 successful primer pairs) based on agarose gel electrophoresis.<br />
Polymerase Chain Reaction Amplification (PCR) is one of the most actively used techniques in molecular biology. In recent years, PCR has been increasingly used for gene expression detection or quantification. It is a more convenient method in gene expression studies comparing to other techniques, such as Northern Blot. One common problem in PCR is the non-specific amplifications of other gene products because cDNAs libraries of thousand of genes are often used as PCR templates.<br />
Therefore, we need to carefully design PCR primers that specifically amplify the genes of interest. Unfortunately, most available primer design programs only focus on primer chemical properties, such as melting temperature, GC content, secondary structure, etc. Little emphasis is given to primer mispriming to other genes. In contrast, all primers in PrimerBank were carefully designed to ensure gene specificity.<br />
Sponsors: PrimerBank is funded by the Massachusetts General Hospital<br />
nif-0000-21333
Resource:PrimerBank
2011-05-18T00:00:00
PrimerBank
Resource
Synonym
ModifiedDate
Label
Temperature
Symbol
Sequence
Secondary structure
Reaction
Quantification
Protein
Primer pair
Primer
Pcr
Mouse
Molecular probe and primer databases
Molecular biology
Melting
Information
Human
Detection
Chemical
Amplification
Algorithm
Agarose
Gene
Gel
Gc content
Expression
Electrophoresis
Keywords
Harvard University; Massachusetts; USA
Is part of
Id
Web accessible database
Has role
Definition
DefiningCitation
CurationStatus